Steroid receptors, including progesterone (PGR), androgen (AR) and glucocorticoid receptors (GR), are transcription factors that share a canonical DNA binding sequence (nuclear receptor response element – NRE). In the ovary, PGR is the key determinant of ovulation and regulates the expression of ovulatory genes. However, the roles of AR and GR in ovulation remain largely unclear. Through qPCR, Western blot and immunofluorescence we showed that all three steroid receptors were present in granulosa cells during mid-ovulation, where PGR largely exerts influence as a transcription factor. To elucidate the relationship between steroid receptors and their impact on ovulatory gene expression, we characterised and contrasted the chromatin binding profiles of PGR, AR and GR in granulosa cells using ChIP-seq. Ovulatory hormone treatment induced PGR and GR chromatin binding while AR action was largely repressed. Interestingly, while AR showed a strong binding preference to the canonical NRE sequence, PGR and GR instead favoured non-canonical motifs corresponding to other transcription factor families, such as AP-1, CEBP and RUNX. Comparative analysis between PGR, AR and GR ChIP-seq showed that two-thirds of PGR binding sites were co-bound by AR and/or GR, indicating substantial interaction between all three steroid receptors. Importantly, the strong proximal promoter binding preference of PGR, which is a unique property of PGR in granulosa cells, was associated with constitutively chromatin-bound AR and GR, suggesting a role for AR and GR in sustaining chromatin accessibility and the recruitment of PGR to target promoters. Overall, in addition to distinct roles in granulosa cells, both AR and GR contribute to the chromatin binding mechanism of PGR. Such relationship is a crucial but largely under-appreciated mechanism that helps to explain how steroid receptors cooperatively mediate gene expression during ovulation.